Our Continuous (kinetic) format uses Fluorescence Intensity, with an Ex/Em wavelength of 360/485-505 nm and allows kinetic monitoring of the activity of a kinase or phosphatase from the same add and read reaction without manual timepoints.
Our innovative Red/Endpoint format uses Europium (Eu3+) with time-resolved fluorescence (TRF) and an Ex/Em wavelength of 360/610-620 nm. The >600 nm wavelength and the ability to use a 100 usec delay, eliminates compound autofluorescence while using a small and minimally hydrophobic fluorophore. Both formats can be performed with the same optimized Sox-sensors, and assays can be run at any ATP concentration.
Assay Kits include all required reagents besides the kinase, allowing 96 reactions (using 50 μL as a final reaction volume in a half-area 96-well plate and 10 μM final substrate) or 192 reactions (using 25 μL as a final reaction volume in a low-volume 384-well plate and 10 μM final substrate).
Bulk Sensor pricing starts at 1 mg net and on average, allows for 1,200 assays using 25 μL as a final reaction volume in a low-volume 384-well plate and 10 μM final substrate). Please contact us about volume discounts.
Custom Assay Development Services are performed under a Master Service Agreement. Custom recombinant assay development has three milestones, and crude cell lysate and tissue lysate assay development has a total of five Milestones. Please visit our Services page to learn more.
Custom Testing Services include kinase profiling and IC50, Ki, kinact/KI, Residence Time and Mechanism of Action (MOA) determinations. Learn more on our Services page.