Protein Phosphatases

To measure the activity of Protein Serine/Threonine or Tyrosine Phosphatases, AssayQuant® offers optimized Sox-based phosphopeptide substrates as part of the PhosphoSens® product line. These sensors undergo a loss of fluorescence signal upon dephosphorylation.

As with PhosphoSens® Sox-based assays for Protein Kinases, the assays for Protein Phosphatases can be performed either kinetically using fluorescence intensity (FI, Ex360/Em485) or as a PhosphoSens®-Red/endpoint format using Europium and time-resolved fluorescence (TRF, Ex360/Em620).

See Sox Technology Sensors and Resources tabs for data by target phosphatase

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Target Enzyme
AssayQuant® Cys-Sox-based Sensors (Substrates)
Enzymes
Source
Sox-based Substrate Name
Substrate MW (Daltons)
Reaction Rate (RFU/pmole kinase/min)
Action
SignalChem (P02-20BG)
1219.29
Very Strong Assay: >1000 RFU/pmole kinase/min
SignalChem (P07-20G)
1219.29
Very Strong Assay: >1000 RFU/pmole kinase/min
PPP2R2A
Details >
BPS (30056)
1219.29
Very Strong Assay: >1000 RFU/pmole kinase/min
BPS (30009)
2151.77
Very Strong Assay: >1000 RFU/pmole kinase/min
BPS (79018)
2151.77
Very Strong Assay: >1000 RFU/pmole kinase/min
R&D Systems (1878-SH)
2151.77
Very Strong Assay: >1000 RFU/pmole kinase/min

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