Protein Kinase Assays

Our validated assays are a simple yet powerful way to measure the activity of protein kinases using a homogeneous and continuous (kinetic) format, where the fluorescence signal is directly proportional to the amount of phosphorylated product. These assays are ideal for elucidating drug mechanism of action and are increasingly being applied across the discovery and drug development workflow to better understand the biology and to address the challenges and opportunities for next-generation protein kinase inhibitors or activators. If you don't see the assay you need, contact us today since we may have an assay for your target in development or we'll develop a custom solution for you.

AssayQuant® is developing multiple formats for measuring the activity of protein kinases, including:

PhosphoSens®: CSox- or Sox-based substrates for use with recombinant or purified kinases, including immunoprecipitation-based kinase assays. Our assays are all non-radioactive, eliminating health risk from exposure to radioactive [32P]-ATP and the costs of radioactive waste disposal, while allowing for efficient kinetic monitoring of the kinase reaction to provide more information with less effort and no additional cost.

PhosphoSens®-Lysate: This product line is currently in development, but initial feasibility has been established (See Peterson et al. 2014 and references therein). These products use CSox-based substrates with high-selectivity for the target kinase, allowing activity to be monitored in crude cell or tissue lysates, providing the same easy-to-use format but with complex samples. Please inquire about current progress or our Custom Assay Development Services.

PhosphoSens®-Red: Excitation Max 360 nm, Emission Max 620 nm, allows the Sox technology to be run as an endpoint format, where the much higher emission wavelength and time-resolved fluorescence detection avoids interference from compound autofluorescence.

See Sox Technology Sensors and Resources tabs for data by target kinase

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Target Enzyme
AssayQuant® Cys-Sox-based Sensors (Substrates)
Enzymes
Source
Sox-based Substrate Name
Substrate MW (Daltons)
Reaction Rate (RFU/pmole kinase/min)
Action
Carna (08-001)
1673.75
Very Strong Assay: >1000 RFU/pmole kinase/min
ABL1 (E255K)
Details >
Carna (08-094)
1673.75
Very Strong Assay: >1000 RFU/pmole kinase/min
ABL1 (T315I)
Details >
Carna (08-093)
1673.75
Very Strong Assay: >1000 RFU/pmole kinase/min
Carna (08-102)
1673.75
Very Strong Assay: >1000 RFU/pmole kinase/min
Carna (01-101)
1488.4
Very Strong Assay: >1000 RFU/pmole kinase/min
Carna (01-102)
1488.4
Very Strong Assay: >1000 RFU/pmole kinase/min
Carna (01-103)
1488.4
Very Strong Assay: >1000 RFU/pmole kinase/min
Carna (08-518)
1384.61
Very Strong Assay: >1000 RFU/pmole kinase/min
ALK (1196M G1202R)
Details >
SignalChem (A19-12NG)
1384.61
Very Strong Assay: >1000 RFU/pmole kinase/min
ALK (C1156Y)
Details >
Carna (08-530)
1384.61
Very Strong Assay: >1000 RFU/pmole kinase/min
ALK (F1174L)
Details >
Carna (08-519)
1384.61
Very Strong Assay: >1000 RFU/pmole kinase/min
ALK (G1202R)
Details >
Carna (08-544)
1384.61
Very Strong Assay: >1000 RFU/pmole kinase/min
ALK (G1269A)
Details >
Carna (08-537)
1384.61
Very Strong Assay: >1000 RFU/pmole kinase/min
ALK (L1196M)
Details >
Carna (08-529)
1384.61
Very Strong Assay: >1000 RFU/pmole kinase/min
ALK (R1275Q)
Details >
Carna (08-520)
1384.61
Very Strong Assay: >1000 RFU/pmole kinase/min

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